NIH 3T3

The name of the pictureThe name of the pictureThe name of the pictureClash Royale CLAN TAG#URR8PPP






NIH 3T3細胞。可見其形態與普通的成纖維細胞類似，爲梭形


NIH 3T3，亦稱3T3，是一個由紐約大學醫學院病理學系的乔治·托达洛（George Todaro）以及哈沃德·格林（Howard Green）兩名研究人員於1962年構建的永生化細胞系[1]。NIH 3T3細胞的來源是NIH瑞士小鼠（NIH Swiss mice）的胚胎成纖維細胞。其名稱中的3T3指該細胞在建系過程中每3天傳代（transfer）一次，每次接種3 x 105個細胞。在傳代20-30次後，兩名成功人員成功建立了今天的NIH 3T3細胞系[2][3]。




目录





  • 1 細胞系的建立


  • 2 特性


  • 3 參見


  • 4 參考




細胞系的建立


3T3細胞由紐約大學醫學院病理學系的兩名學者乔治·托达洛（George Todaro）以及哈沃德·格林（Howard Green）於1962年構建。該細胞的來源是NIH瑞士小鼠（NIH Swiss mice）的胚胎成纖維細胞。他們構建細胞系時，在原代培養後，每3天傳代（transfer）一次，並以3 x 105個細胞每20cm2的密度將細胞鋪到新的組織培養皿中。在傳代20-30次後，他們發現一部分成纖維細胞的生長速度開始加快，隨後，即使再經過多次傳代，這些細胞還是能繼續生長分裂（即這些細胞已經跨越了海弗里克極限，進入了永生化狀態）。他們在1963年報告了這一成果。隨後，該細胞的名稱前面加上了NIH（美國國立衛生研究院）的名字，稱爲NIH 3T3[1]。



特性


NIH 3T3細胞通常於添加10%胎牛血清（FBS）的DMEM培養基中培養，根據情況可以向培養基中添加一定量的抗生素，以預防細菌污染。該細胞的細胞週期大約爲18小時[4]。傳代時使用胰蛋白酶消化即可。NIH細胞爲貼壁生長，有接觸抑制現象。在顯微鏡下，可以觀察到培養皿的NIH細胞呈梭形[3]。


NIH 3T3細胞的轉染效率較高，細胞生物學研究者常常將其選作研究模型[3]。對NIH 3T3的基因組分析表明，NIH 3T3細胞爲非整倍體，研究表明，NIH 3T3細胞的染色體數範圍大致在52條到82條之間，大部分（超過70%）NIH 3T3細胞中含有70到78條染色體（正常二倍體小鼠的染色體數爲40）[5]。



參見


  • HeLa細胞

  • 293細胞


參考




  1. ^ 1.01.1 TODARO GJ, GREEN H. Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines.. J Cell Biol. 1963 May; 17():299-313. 


  2. ^ Howard Green. The Story of 3T3 Cells: A Voyage of Discovery Without an Itinerary. 中國科學院計算研究所. [2017-07-01]. 


  3. ^ 3.03.13.2 NIH 3T3 Cell Line Origins, Characteristics, Expression, and Cytogenetics. NIH3T3.com. [2017-07-01]. 


  4. ^ 3T3 cells. ATCC. [2017-07-01]. 


  5. ^ Christine Leibiger; Thomas Liehr; 等. First Molecular Cytogenetic High Resolution Characterization of the NIH 3T3 Cell Line by Murine Multicolor Banding. J Histochem Cytochem. 2013 Apr; 61(4): 306–312. 





维基共享资源中相关的多媒体资源：NIH 3T3
-

Popular posts from this blog

California gubernatorial recall election

Image
Clash Royale CLAN TAG #URR8PPP California gubernatorial recall election, 2003 ← 2002 October 7, 2003 2006 →   Candidate Arnold Schwarzenegger Cruz Bustamante Tom McClintock Party Republican Democratic Republican Popular vote 4,206,284 2,724,874 1,161,287 Percentage 48.6% 31.5% 13.4% County Results Schwarzenegger :       40–50%       50–60%       60–70% Bustamante :       40-50%       50–60%       60–70% Governor before election Gray Davis Democratic Elected Governor Arnold Schwarzenegger Republican Elections in California Federal government Presidential elections 1852 1856 1860 1864 1868 1872 1876 1880 1884 1888 1892 1896 1900 1904 1908 1912 1916 1920 1924 1928 1932 1936 1940 1944 1948 1952 1956 1960 1964 1968 1972 1976 1980 1984 1988 1992 1996 2000 2004 2008 2012 2016 Pri...
Read more

Telugu cinema

Image
Clash Royale CLAN TAG #URR8PPP Telugu cinema Prasads Multiplex, Hyderabad No. of screens 2809 screens in Andhra Pradesh and Telangana states of India [1] Main distributors Arka Media Works Suresh Productions Sri Venkateswara Creations Geetha Arts 14 Reels Entertainment PVP Cinema Prasad Art Pictures Usha Kiron Movies Vyjayanthi Movies Annapurna Studios Indian cinema Assamese Bengali (Tollywood) Bhojpuri Badaga Bihari Chhattisgarhi Deccani (Deccanwood) Dogri Gujarati (Gollywood/Dhollywood) Haryanvi Hindi (Bollywood) Jharkhandi (Jollywood) Kashmiri Kannada (Sandalwood) Konkani Kosli Kutchi Malayalam (Mollywood) Marathi Meitei Odia Punjabi Rajasthani Sanskrit Santali Tamil (Kollywood) Telugu (Tollywood) Tulu (Coastalwood) Telugu cinema , also known by its sobriquet Tollywood , is the segment of Indian cinema dedicated to the production of motion pictures in the Telugu language, based in Film Nagar, a neighborhood of Hyderabad, Telangana. [4] Since 1909, film maker Raghupathi Venkaiah ...
Read more

The Internationale

Image
Clash Royale CLAN TAG #URR8PPP For the Billy Bragg album, see The Internationale (album). For the Brainiac EP, see Internationale (EP). body.skin-minerva .mw-parser-output table.infobox caption.infobox-anthem-captionwhite-space:nowrap "The Internationale" "L'Internationale", original French version International anthem of The Communist movement, The Anarchist movement, The Socialist movement, The Democratic Socialist movement, The Social Democratic movement Also known as L'Internationale (French) Lyrics Eugène Pottier, 1871 Music Pierre De Geyter, 1888 Adopted 1890s Audio sample "The Internationale" (instrumental) file help v t e " The Internationale " (French: "L'Internationale" ) is a left-wing anthem. It has been a standard of the socialist movement since the late nineteenth century, when the Second International adopted it as its official anthem. The title arises from the "First International", an alliance of...
Read more